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KD-Validated Anti-TRK fused gene Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TRK fused gene Rabbit Monoclonal Antibody AGI1709
    Western blotting analysis using anti-TFG antibody (Cat#AGI1709). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TFG antibody (Cat#AGI1709, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-TRK fused gene Rabbit Monoclonal Antibody AGI1709
    Western blotting analysis using anti-TFG antibody (Cat#AGI1709). TFG expression in wild type (WT) and TFG shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TFG antibody (Cat#AGI1709, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-TRK fused gene Rabbit Monoclonal Antibody AGI1709
    Flow cytometric analysis of TFG expression in HepG2 cells using anti-TFG antibody (Cat#AGI1709, 1:2,000). Green, isotype control; red, TFG.
    detail
  • ICC - KD-Validated Anti-TRK fused gene Rabbit Monoclonal Antibody AGI1709
    Immunocytochemical staining of HepG2 cells with anti-TFG antibody (Cat#AGI1709, 1:1,000). Nuclei were stained blue with DAPI; TFG  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q92734
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB2115
Calculated MW Predicted, 43 kDa , observed , 55 kDa
Gene Name TFG
Aliases TFG; Trafficking From ER To Golgi Regulator; SPG57; TF6; TRK-Fused Gene Protein; Protein TFG; FLJ36137; TRK-Fused Gene; TRKT3 Oncogene; TRK-Fused;HMSNP; TRKT3
Immunogen A synthesized peptide derived from human TRK fused gene
Additional Information
Gene ID 10342
Other Names Protein TFG, TRK-fused gene protein, TFG
Protein Information
Name TFG
Function Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed:23479643, PubMed:27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:21478858).
Cellular Location Endoplasmic reticulum. Note=Localizes to endoplasmic reticulum exit site (ERES), also known as transitional endoplasmic reticulum (tER) (PubMed:21478858, PubMed:27813252).
Tissue Location Ubiquitous.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1709
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