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KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI1805
    Western blotting analysis using anti-cyclin B1 antibody (Cat#AGI1805). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin B1 antibody (Cat#AGI1805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI1805
    Western blotting analysis using anti-cyclin B1 antibody (Cat#AGI1805). Cyclin B1 expression in wild-type (WT) and cyclin B1 (CCNB1) shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin B1 antibody (Cat#AGI1805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI1805
    Flow cytometric analysis of Cyclin B1 expression in HeLa cells using anti-Cyclin B1 antibody (Cat#AGI1805, 1:2,000). Green, isotype control; red, Cyclin B1.
    detail
  • ICC - KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody AGI1805
    Immunocytochemical staining of Hela cells with cyclin B1 antibody (Cat#AGI1805, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P14635
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB4305
Calculated MW Predicted, 48 kDa, observed, 55 kDa
Gene Name CCNB1
Aliases CCNB1; Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin-B1; G2/Mitotic-Specific Cyclin B1
Immunogen Recombinant protein of mouse Cyclin B1 (UniProt Entry: P24860)
Additional Information
Gene ID 891
Other Names G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Protein Information
Name CCNB1
Synonyms CCNB
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1805
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