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KD-Validated Anti-Epsin 2 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Epsin 2 Mouse Monoclonal Antibody AGI2032
    Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-Epsin 2 Mouse Monoclonal Antibody AGI2032
    Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Epsin 2 expression in wild type (WT) and epsin 2 (EPN2) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-Epsin 2 Mouse Monoclonal Antibody AGI2032
    Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#63852, 1:1,000). Green, isotype control; red, Epsin 2.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O95208
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB8190
Calculated MW Predicted, 68 kDa, observed, 68 kDa
Gene Name EPN2
Aliases EPN2; Epsin 2; KIAA1065; EHB21; EPS-15-Interacting Protein 2; Eps15 Binding Protein; Epsin-2
Immunogen Recombinant protein of human EPN2
Additional Information
Gene ID 22905
Other Names Epsin-2, EPS-15-interacting protein 2, EPN2, KIAA1065
Protein Information
Name EPN2
Synonyms KIAA1065
Function Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Cellular Location Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle. Note=In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex
Tissue Location Highest expression is found in brain. Detected at lower levels in lung and liver.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2032
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