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KD-Validated Anti-ACACA Rabbit Polyclonal Antibody

Rabbit polyclonal antibody

     
  • WB - KD-Validated Anti-ACACA Rabbit Polyclonal Antibody AGI2142
    Western blotting analysis using anti-ACACA antibody (Cat#AGI2142). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ACACA antibody (Cat#AGI2142, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-ACACA Rabbit Polyclonal Antibody AGI2142
    Western blotting analysis using anti-ACACA antibody (Cat#AGI2142). ACACA expression in wild-type (WT) and ACACA shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ACACA antibody (Cat#AGI2142, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13085
Reactivity Rat, Human, Mouse
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW Predicted, 266 kDa, observed, 250 kDa
Gene Name ACACA
Aliases ACACA; Acetyl-CoA Carboxylase Alpha; ACC-Alpha; ACC1; ACCA; Acetyl-Coenzyme A Carboxylase Alpha; Acetyl-CoA Carboxylase 1; ACACalpha; ACCalpha; Acac1; HACC1; ACAC; ACC; EC 6.4.1.2; ACACAD
Immunogen A synthesized peptide derived from human ACC alpha
Additional Information
Gene ID 31
Other Names Acetyl-CoA carboxylase 1, ACC1, 6.4.1.2, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha, ACACA (HGNC:84), ACAC, ACC1, ACCA
Protein Information
Name ACACA (HGNC:84)
Synonyms ACAC, ACC1, ACCA
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl- CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q5SWU9}
Tissue Location Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2142
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