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KD-Validated Anti-EEF2 Rabbit Polyclonal Antibody

Rabbit polyclonal antibody

     
  • WB - KD-Validated Anti-EEF2 Rabbit Polyclonal Antibody AGI2145
    Western blotting analysis using anti-EEF2 antibody (Cat#AGI2145). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EEF2 antibody (Cat#AGI2145, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-EEF2 Rabbit Polyclonal Antibody AGI2145
    Western blotting analysis using anti-EEF2 antibody (Cat#AGI2145). EEF2 expression in wild-type (WT) and EEF2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EEF2 antibody (Cat#AGI2145, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P13639
Reactivity Rat, Human, Mouse
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW Predicted, 95 kDa, observed, 95 kDa
Gene Name EEF2
Aliases EEF2; Eukaryotic Translation Elongation Factor 2; EEF-2; EF2; Polypeptidyl-TRNA Translocase; Elongation Factor 2; EF-2; Epididymis Secretory Sperm Binding Protein; C 3.6.5.-; SCA26
Immunogen A synthesized peptide derived from human EEF2
Additional Information
Gene ID 1938
Other Names Elongation factor 2, EF-2, 3.6.5.-, EEF2, EF2
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C- terminal cleavage product.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2145
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