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KD-Validated Anti-ATG5 Rabbit Polyclonal Antibody

Rabbit polyclonal antibody

     
  • WB - KD-Validated Anti-ATG5 Rabbit Polyclonal Antibody AGI2155
    Western blotting analysis using anti-ATG5 antibody (Cat#AGI2155). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG5 antibody (Cat#AGI2155, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-ATG5 Rabbit Polyclonal Antibody AGI2155
    Western blotting analysis using anti-ATG5 antibody (Cat #AGI2155). ATG5 expression in wild-type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG5 antibody (Cat #AGI2155, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9H1Y0
Reactivity Human
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW Predicted, 32 kDa, observed, 55 kDa
Gene Name ATG5
Aliases ATG5; Autophagy Related 5; ASP; HAPG5; APG5L; APG5; Apoptosis-Specific Protein; Autophagy Protein 5; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae); ATG5 Autophagy Related 5 Homolog; APG5 Autophagy 5-Like; APG5-LIKE; APG5-Like; SCAR25
Immunogen A synthesized peptide derived from human ATG5
Additional Information
Gene ID 9474
Other Names Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5 (HGNC:589), APG5L, ASP
Protein Information
Name ATG5 (HGNC:589)
Synonyms APG5L, ASP
Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3- like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Also localizes to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. Under starved conditions, the ATG12-ATG5-ATG16L1 complex is translocated to phagophores driven by RAB33B (PubMed:32960676). {ECO:0000250, ECO:0000269|PubMed:32960676}
Tissue Location Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2155
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