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KD-Validated Anti-ASRGL1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-ASRGL1 Mouse Monoclonal Antibody AGI2181
    Western blotting analysis using anti-ASRGL1 antibody (Cat#AGI2181). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASRGL1 antibody (Cat#AGI2181, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-ASRGL1 Mouse Monoclonal Antibody AGI2181
    Western blotting analysis using anti-ASRGL1 antibody (Cat#AGI2181). ASRGL1 expression in wild-type (WT) and ASRGL1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ASRGL1 antibody (Cat#AGI2181, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-ASRGL1 Mouse Monoclonal Antibody AGI2181
    Flow cytometric analysis of ASRGL1 expression in HeLa cells using anti-ASRGL1 antibody (Cat#AGI2181, 1:2,000). Green, isotype control; red, ASRGL1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q7L266
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2a
Clone Names 24GB14265
Calculated MW Predicted, 32 kDa, observed, 40 kDa
Gene Name ASRGL1
Aliases ASRGL1; Asparaginase And Isoaspartyl Peptidase 1; ALP; ALP1; Isoaspartyl Peptidase/L-Asparaginase; Asparaginase-Like 1 Protein; L-Asparagine Amidohydrolase; Asparaginase-Like Protein 1; Beta-Aspartyl-Peptidase; Isoaspartyl Dipeptidase; Asparaginase Like 1; EC 3.5.1.1; FLJ22316; CRASH; Testis Secretory Sperm-Binding Protein Li 242mP; L-Asparaginase; EC 3.4.19.5
Immunogen Recombinant protein of human ASRGL1
Additional Information
Gene ID 80150
Other Names Isoaspartyl peptidase/L-asparaginase, 3.4.19.5, 3.5.1.1, Asparaginase-like protein 1, Beta-aspartyl-peptidase, Isoaspartyl dipeptidase, L-asparagine amidohydrolase, Isoaspartyl peptidase/L-asparaginase alpha chain, Isoaspartyl peptidase/L-asparaginase beta chain, ASRGL1, ALP, CRASH
Protein Information
Name ASRGL1
Synonyms ALP, CRASH
Function Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp- L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
Cellular Location Cytoplasm. Note=Midpiece of sperm tail
Tissue Location Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level). Over- expressed in uterine, mammary, prostatic and ovarian carcinoma
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2181
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