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KD-Validated Anti-POLDIP2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-POLDIP2 Rabbit Monoclonal Antibody AGI2261
    Western blotting analysis using anti-POLDIP2 antibody (Cat#AGI2261). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLDIP2 antibody (Cat#AGI2261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-POLDIP2 Rabbit Monoclonal Antibody AGI2261
    Western blotting analysis using anti-POLDIP2 antibody (Cat#AGI2261). POLDIP2 expression in wild-type (WT) and POLDIP2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLDIP2 antibody (Cat#AGI2261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9Y2S7
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB4415
Calculated MW Predicted, 42 kDa; observed, 35 kDa
Gene Name POLDIP2
Aliases POLDIP2; DNA Polymerase Delta Interacting Protein 2; PDIP38; Polymerase (DNA-Directed), Delta Interacting Protein 2; 38 KDa DNA Polymerase Delta Interaction Protein; Polymerase Delta-Interacting Protein 2; DKFZP586F1524; POLD4; P38; Polymerase (DNA) Delta Interacting Protein 2; Polymerase Delta Interacting Protein 38
Immunogen A synthesized peptide derived from human POLDIP2
Additional Information
Gene ID 26073
Other Names Polymerase delta-interacting protein 2, 38 kDa DNA polymerase delta interaction protein, p38, POLDIP2 {ECO:0000303|PubMed:26984527, ECO:0000312|HGNC:HGNC:23781}
Protein Information
Name POLDIP2 {ECO:0000303|PubMed:26984527, ECO:0000312|HGNC:HGNC:23781}
Function Involved in DNA damage tolerance by regulating translesion synthesis (TLS) of templates carrying DNA damage lesions such as 8oxoG and abasic sites (PubMed:24191025). May act by stimulating activity of DNA polymerases involved in TLS, such as PRIMPOL and polymerase delta (POLD1) (PubMed:24191025, PubMed:26984527).
Cellular Location Mitochondrion matrix. Nucleus Note=Mainly localizes to the mitochondrial matrix; a small fraction localizes in the nucleus.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2261
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