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KD-Validated Anti-CRKII Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CRKII Rabbit Monoclonal Antibody AGI2377
    Western blotting analysis using anti-CRKII antibody (Cat#AGI2377). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRKII antibody (Cat#AGI2377, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CRKII Rabbit Monoclonal Antibody AGI2377
    Western blotting analysis using anti-CRKII antibody (Cat#AGI2377). CRKII expression in wild type (WT) and CRKII shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CRKII antibody (Cat#AGI2377, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CRKII Rabbit Monoclonal Antibody AGI2377
    Flow cytometric analysis of CRKII expression in HepG2 cells using CRKII antibody (Cat#AGI2377, 1:2,000). Green, isotype control; red, CRKII.
    detail
  • ICC - KD-Validated Anti-CRKII Rabbit Monoclonal Antibody AGI2377
    Immunocytochemical staining of HepG2 cells with CRKII antibody (Cat#AGI2377, 1:1,000). Nuclei were stained blue with DAPI; CRKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P46108
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1405
Calculated MW Predicted, 34 kDa; Observed, 35 kDa
Gene Name CRK
Aliases CRK; CRK Proto-Oncogene, Adaptor Protein; V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog; Adapter Molecule Crk; Proto-Oncogene C-Crk; P38; V-Crk Sarcoma Virus CT10 Oncogene-Like Protein; CRKII
Immunogen A synthesized peptide derived from human CRKII
Additional Information
Gene ID 1398
Other Names Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK
Protein Information
Name CRK
Function Involved in cell branching and adhesion mediated by BCAR1- CRK-RAPGEF1 signaling and activation of RAP1.
Cellular Location Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2377
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