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KO Validated Anti-CREB1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KO Validated Anti-CREB1 Mouse Monoclonal Antibody AGI2413
    Western blotting analysis using anti-CREB1 antibody (Cat#71152). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CREB1, cAMP responsive element binding protein 1.
    detail
  • WB - KO Validated Anti-CREB1 Mouse Monoclonal Antibody AGI2413
    Western blotting analysis using anti-CREB1 antibody (Cat#71152). CREB1 expression in wild type (WT) and CREB1 knockout (KO) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CREB1 antibody (Cat#71152, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P16220
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1 kappa
Clone Names 25GB565
Calculated MW Predicted, 35 kDa, observed, 43 kDa
Gene Name CREB1
Aliases CREB1; CAMP Responsive Element Binding Protein 1; Cyclic AMP-Responsive Element-Binding Protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein CREB; Cyclic Adenosine 3',5'-Monophosphate Response Element Binding Protein; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB
Immunogen Recombinant protein of human CREB
Additional Information
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2413
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