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ATP6V1A1 / ATP6V1A Antibody (clone 4F5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P38606 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4F5 |
| Calculated MW | 68kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 523 |
|---|---|
| Other Names | V-type proton ATPase catalytic subunit A, V-ATPase subunit A, 3.6.3.14, V-ATPase 69 kDa subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha, ATP6V1A, ATP6A1, ATP6V1A1, VPP2 |
| Target/Specificity | Human ATP6V1A |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | ATP6V1A1 / ATP6V1A Antibody (clone 4F5) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP6V1A |
|---|---|
| Synonyms | ATP6A1, ATP6V1A1, VPP2 |
| Function | Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857). |
| Cellular Location | Cytoplasm. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P50516}. Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250|UniProtKB:P31404}; Peripheral membrane protein. Lysosome {ECO:0000250|UniProtKB:P50516} Note=Co-localizes with WFS1 in the secretory granules in neuroblastoma cell lines. |
| Tissue Location | High expression in the skin. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
References
van Hille B.,et al.J. Biol. Chem. 268:7075-7080(1993).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
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