Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Anti-nNOS Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P29475 |
|---|---|
| Host | Rabbit |
| Clonality | Rabbit Polyclonal |
| Isotype | IgG |
| Calculated MW | 160970 Da |
| Gene ID | 4842 |
|---|---|
| Other Names | neuronal nitric oxide synthase, BNOS, Constitutive NOS, IHPS1, N-NOS, Nitric oxide synthase 1, NOS type I, NC-NOS |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Anti-nNOS Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, [[URL:https://www.novusbio.com/research-areas/neuroscience/neurotransmission.html]][[Caption:neurotransmission]] and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, [[URL:https://www.novusbio.com/common-name/nnos]][[Caption:nNOS]] (type I) and endothelial cell NOS, [[URL:https://www.novusbio.com/common-name/enos]][[Caption:eNOS]] (type III); along with the inducible isoform, [[URL:https://www.novusbio.com/common-name/inos]][[Caption:iNOS]] (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors [[URL:https://www.novusbio.com/common-name/calmodulin]][[Caption:calmodulin]], nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), [[URL:https://www.novusbio.com/common-name/heme]][[Caption:heme]], and [[URL:https://www.novusbio.com/common-name/tetrahydrobiopterin]][[Caption:tetrahydrobiopterin]] (1).
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and [[URL:https://www.novusbio.com/research-areas/immunology/chemokines-cytokines]][[Caption:cytokines]] such as [[URL:https://www.novusbio.com/common-name/ifn-gamma]][[Caption:IFN-gamma]], [[URL:https://www.novusbio.com/common-name/tnf-alpha]][[Caption:TNF]], [[URL:https://www.novusbio.com/common-name/il-1-beta-il-1f2]][[Caption:IL-1]] and [[URL:https://www.novusbio.com/common-name/il-2]][[Caption:IL-2]]. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and [[URL:https://www.novusbio.com/research-areas/lipid-and-metabolism-diabetes-research.html]][[Caption:type 2 diabetes]]. Acting as a critical mediator of [[URL:https://www.novusbio.com/research-areas/immunology/inflammation]][[Caption:inflammation]] and [[URL:https://www.novusbio.com/research-areas/apoptosis]][[Caption:apoptosis]], iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
References
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
