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>   home   >   Products   >   Primary Antibodies   >   CEBPE Antibody (C-term)   

CEBPE Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CEBPE Antibody (C-term) AP14112b
    Western blot analysis of CEBPE (arrow) using rabbit polyclonal CEBPE Antibody (C-term) (Cat. #AP14112b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CEBPE gene.
  • WB - CEBPE Antibody (C-term) AP14112b
    CEBPE Antibody (C-term) (Cat. #AP14112b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CEBPE antibody detected the CEBPE protein (arrow).
  • WB - CEBPE Antibody (C-term) AP14112b
    CEBPE Antibody (C-term) (Cat. #AP14112b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CEBPE antibody detected the CEBPE protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15744
Other Accession NP_001796.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30603 Da
Antigen Region 203-232 aa
Additional Information
Gene ID 1053
Other Names CCAAT/enhancer-binding protein epsilon, C/EBP epsilon, CEBPE
Target/Specificity This CEBPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the C-terminal region of human CEBPE.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCEBPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CEBPE
Function Transcriptional activator (PubMed:26019275). C/EBP are DNA- binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).
Cellular Location Nucleus
Tissue Location Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Citations (0)

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Background

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.

References

Prasad, R.B., et al. Blood (2009) In press :
Papaemmanuil, E., et al. Nat. Genet. 41(9):1006-1010(2009)
Bedi, R., et al. Blood 113(2):317-327(2009)
Cloutier, A., et al. J. Immunol. 182(1):563-571(2009)
Matsushita, H., et al. Oncogene 27(53):6749-6760(2008)

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Discontinued
Cat# AP14112b
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