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FAU Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FAU Antibody (N-term) AP1600a
    Western blot analysis of FUBI Antibody (N-term) polyclonal antibody(Cat.#AP1600a)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FUBI gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - FAU Antibody (N-term) AP1600a
    Formalin-fixed and paraffin-embedded human colon carcinoma reacted with FUBI Antibody (N-term)(Cat.#AP1600a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P35544
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 1-30 aa
Additional Information
Other Names Ubiquitin-like protein FUBI, FAU
Target/Specificity This FAU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FAU.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAU Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

FUBI is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It is a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome.

References

Rossman, T.G., et al., Oncogene 22(12):1817-1821 (2003).
Kenmochi, N., et al., Genome Res. 8(5):509-523 (1998).
Vladimirov, S.N., et al., Eur. J. Biochem. 239(1):144-149 (1996).
Kas, K., et al., Genomics 17(2):387-392 (1993).
Michiels, L., et al., Oncogene 8(9):2537-2546 (1993).

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$ 182.50
$ 70.00
Cat# AP1600a
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