MCF2L Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O15068 |
Other Accession | NP_001106203.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 128109 Da |
Antigen Region | 1049-1075 aa |
Gene ID | 23263 |
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Other Names | Guanine nucleotide exchange factor DBS, DBL's big sister, MCF2-transforming sequence-like protein, MCF2L, KIAA0362 |
Target/Specificity | This MCF2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1075 amino acids from the C-terminal region of human MCF2L. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MCF2L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MCF2L |
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Synonyms | KIAA0362, OST {ECO:0000303|PubMed:151576 |
Function | Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42 (PubMed:15157669). |
Cellular Location | [Isoform 5]: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm {ECO:0000250|UniProtKB:Q64096}. Cell membrane {ECO:0000250|UniProtKB:Q64096}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q64096}; Cytoplasmic side {ECO:0000250|UniProtKB:Q64096} |
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Background
Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
Ieguchi, K., et al. J. Biol. Chem. 282(32):23296-23305(2007)
Yang, Q., et al. BMC Med. Genet. 8 SUPPL 1, S12 (2007) :
Kostenko, E.V., et al. J. Biol. Chem. 280(4):2807-2817(2005)
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