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RNF6 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9Y252 |
| Other Accession | NP_898865.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 78091 Da |
| Antigen Region | 529-558 aa |
| Gene ID | 6049 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF6, 632-, RNF6, SPG2 |
| Target/Specificity | This RNF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-558 amino acids from the C-terminal region of human RNF6. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNF6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF6 {ECO:0000303|PubMed:10331950, ECO:0000312|HGNC:HGNC:10069} |
|---|---|
| Function | E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover (By similarity). Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity (PubMed:19345326). May also bind DNA and function as a transcriptional regulator (By similarity). Mediates polyubiquitination of QKI in macrophages, leading to its degradation (By similarity). |
| Cellular Location | Nucleus. Cytoplasm. Cell projection, axon {ECO:0000250|UniProtKB:Q9DBU5}. Nucleus, PML body {ECO:0000250|UniProtKB:Q9DBU5}. Note=Localizes to the PML nuclear bodies in Sertoli cells. {ECO:0000250|UniProtKB:Q9DBU5} |
| Tissue Location | Weakly expressed in peripheral blood, spleen, prostate, testis and ovary (PubMed:19345326). According to a report, it is preferentially expressed in testis and ovary and hardly detected in other tissues (PubMed:18368307). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed.
References
Xu, K., et al. Cancer Cell 15(4):270-282(2009)
Zhu, L., et al. Sci. China, C, Life Sci. 51(4):302-307(2008)
Tursun, B., et al. Genes Dev. 19(19):2307-2319(2005)
Lo, H.S., et al. Cancer Res. 62(15):4191-4193(2002)
Lopez, P., et al. Mol. Cell. Biol. 22(10):3488-3496(2002)
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