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PLEC Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PLEC Antibody (C-Term) AP22252b
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PLEC with AP22252b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
  • WB - PLEC Antibody (C-Term) AP22252b
    Anti-PLEC Antibody (C-Term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q15149
Other Accession Q9JI55, Q9QXS1, P30427
Reactivity Human
Predicted Hamster, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 531791 Da
Additional Information
Gene ID 5339
Other Names Plectin, PCN, PLTN, Hemidesmosomal protein 1, HD1, Plectin-1, PLEC, PLEC1
Target/Specificity This PLEC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4241-4275 amino acids from human PLEC.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLEC Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEC
Synonyms PLEC1
Function Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
Cellular Location Cytoplasm, cytoskeleton. Cell junction, hemidesmosome
Tissue Location Widely expressed with highest levels in muscle, heart, placenta and spinal cord
Research Areas
Citations (0)

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Background

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.

References

Liu C.-G.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:4278-4283(1996).
McLean W.H.I.,et al.Genes Dev. 10:1724-1735(1996).
Zhang T.,et al.Genome Res. 14:79-89(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 162.50
$ 54.50
Cat# AP22252b
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