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Anti-Cadherin 23 Antibody

     
  • WB - Anti-Cadherin 23 Antibody AP53803
    Western blot analysis of Cadherin 23 expression in HEK293T (A) whole cell lysates.
    detail
  • IH - Anti-Cadherin 23 Antibody AP53803
    Immunohistochemical analysis of Cadherin 23 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • IF - Anti-Cadherin 23 Antibody AP53803
    Immunofluorescent analysis of Cadherin 23 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC
Primary Accession Q9H251
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 369494 Da
Additional Information
Gene ID 64072
Other Names KIAA1774; KIAA1812; Cadherin-23; Otocadherin
Target/Specificity Recognizes endogenous levels of Cadherin 23 protein.
Dilution WB~~1/500 - 1/1000
IH~~1/50 - 1/200
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CDH23 {ECO:0000303|PubMed:11138009, ECO:0000312|HGNC:HGNC:13733}
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. CDH23 is required for establishing and/or maintaining the proper organization of the stereocilia bundle of hair cells in the cochlea and the vestibule during late embryonic/early postnatal development. It is part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Particularly strong expression in the retina (PubMed:11138009). Found also in the cochlea
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to Cadherin 23

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$ 350.00
Cat# AP53803
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