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Anti-GPR27 Antibody

     
  • WB - Anti-GPR27 Antibody AP53993
    Western blot analysis of GPR27 expression in HEK293T (A), SGC7901 (B), mouse lung (C) whole cell lysates.
    detail
  • IF - Anti-GPR27 Antibody AP53993
    Immunofluorescent analysis of GPR27 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9NS67
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 39818 Da
Additional Information
Gene ID 2850
Other Names SREB1; Probable G-protein coupled receptor 27; Super conserved receptor expressed in brain 1
Target/Specificity Recognizes endogenous levels of GPR27 protein.
Dilution WB~~1/500 - 1/1000
IF~~1/50 - 1/200
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name GPR27
Synonyms SREB1
Function Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed as a 3.0 kb transcript in brain, ovary, testis, heart, prostate and peripheral Leukocytes. Lower levels in pancreas and small intestine. A 2.3 kb transcript was also found in peripheral Leukocytes. In brain regions, detected as a 3.0 kb transcript in all regions tested. Highest levels in the caudate nucleus, putamen, hippocampus and subthalamic nucleus. Lowest level in the cerebellum
Research Areas
Citations (0)
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Background

Rabbit polyclonal antibody to GPR27

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$ 350.00
Cat# AP53993
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