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TMEM85 Polyclonal Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, IHC-F, IF, ICC, E |
|---|---|
| Primary Accession | Q5J8M3 |
| Reactivity | Pig, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 20, 17 KDa |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human TMEM85 |
| Epitope Specificity | 2-100/183 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Membrane. |
| SIMILARITY | Belongs to the TMEM85 family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | May mediate anti-apoptotic activity. |
| Gene ID | 51234 |
|---|---|
| Other Names | ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85, EMC4, TMEM85 |
| Target/Specificity | Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃. |
| Name | EMC4 |
|---|---|
| Synonyms | TMEM85 |
| Function | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242231, PubMed:29809151, PubMed:30415835, PubMed:32439656, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29242231, PubMed:29809151, PubMed:30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151, PubMed:30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29242231, PubMed:29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N- terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Could also be a single-pass transmembrane protein with cytosolic N-terminus and lumenal C-terminus. |
| Tissue Location | Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. |
Research Areas
Citations (0)
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Application Protocols
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$ 385.00
Cat# AP54927
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