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5HT3B receptor Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - 5HT3B receptor Polyclonal Antibody AP58148
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (bs-4289R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • FC - 5HT3B receptor Polyclonal Antibody AP58148
    Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
    Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody (bs-4289R-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
    detail
  • WB - 5HT3B receptor Polyclonal Antibody AP58148
    Sample: 293T Cell (Human) Lysate at 40 ug
    Primary: Anti- 5HT3B receptor (bs-4289R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 50 kD
    Observed band size: 55 kD
    detail
  • WB - 5HT3B receptor Polyclonal Antibody AP58148
    Sample: Lovo Cell (Human) Lysate at 40 ug
    Primary: Anti- 5HT3B receptor (bs-4289R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 50 kD
    Observed band size: 55 kD
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession O95264
Reactivity Rat, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 50292 Da
Additional Information
Gene ID 9177
Other Names 5-hydroxytryptamine receptor 3B, 5-HT3-B, 5-HT3B, Serotonin receptor 3B, HTR3B
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name HTR3B (HGNC:5298)
Function Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation- selective channel complexes, which when activated cause fast, depolarizing responses in neurons.
Cellular Location Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A
Tissue Location Expressed in the brain cortex, in the caudate nucleus, the hippocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta
Research Areas
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$ 350.00
Cat# AP58148
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