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Anti-p47 phox (pS345) Antibody

Rabbit polyclonal antibody to p47 phox (pS345)

     
  • WB - Anti-p47 phox (pS345) Antibody AP60701
    Western blot analysis of p47 phox (pS345) expression in rat lung (A), rat liver (B) whole cell lysates.
    detail
  • IH - Anti-p47 phox (pS345) Antibody AP60701
    Immunohistochemical analysis of p47 phox (pS345) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P14598
Other Accession Q09014
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44682 Da
Additional Information
Gene ID 653361
Other Names NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Target/Specificity Recognizes endogenous levels of p47 phox (pS345) protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p47 phox. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60701
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