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Anti-ITPKA Antibody

Rabbit polyclonal antibody to ITPKA

     
  • WB - Anti-ITPKA Antibody AP60870
    Western blot analysis of ITPKA expression in A549 (A), LO2 (B) whole cell lysates.
    detail
  • IF - Anti-ITPKA Antibody AP60870
    Immunofluorescent analysis of ITPKA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P23677
Other Accession Q8R071
Reactivity Human, Mouse, Rat, Pig, Bovine, Drosophila
Host Rabbit
Clonality Polyclonal
Calculated MW 51009 Da
Additional Information
Gene ID 3706
Other Names Inositol-trisphosphate 3-kinase A; Inositol 145-trisphosphate 3-kinase A; IP3 3-kinase A; IP3K A; InsP 3-kinase A
Target/Specificity Recognizes endogenous levels of ITPKA protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ITPKA (HGNC:6178)
Function Catalyzes the phosphorylation of 1D-myo-inositol 1,4,5- trisphosphate (InsP3) into 1D-myo-inositol 1,3,4,5-tetrakisphosphate and participates to the regulation of calcium homeostasis.
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Expressed in brain..
Research Areas
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ITPKA. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60870
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