SIK2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, ICC, E |
---|---|
Primary Accession | Q9H0K1 |
Other Accession | EAW67148, 38569460 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 103915 Da |
Application Notes | SIK2 antibody can be used for detection of SIK2 by Western blot at 1 µg/mL. |
Gene ID | 23235 |
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Target/Specificity | SIK2; SIK2 antibody is predicted to not cross-react with other SIK protein family members. |
Reconstitution & Storage | SIK2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | SIK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SIK2 |
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Synonyms | KIAA0781 {ECO:0000312|EMBL:BAA34501.3}, |
Function | Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed:23322770, PubMed:26983400). Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators (PubMed:15454081). Phosphorylates EP300 and thus inhibits its histone acetyltransferase activity (PubMed:21084751, PubMed:26983400). In turn, regulates the DNA-binding ability of several transcription factors such as PPARA or MLXIPL (PubMed:21084751, PubMed:26983400). Also plays a role in thymic T-cell development (By similarity). |
Cellular Location | Cytoplasm. Endoplasmic reticulum membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
SIK2 Antibody: Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
References
Horike N, Takemori H, Katoh Y, et al. Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 2003; 278:18440-7.
Screaton RA, Conkright MD, Katoh Y, et al. The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector. Cell 2004; 119:61-74.
Muraoka M, Fukushima A, Viengchareun S, et al. Involvement of SIK2/TORC2 signaling cascade in the regulation of insulin-induced PGC-1alpha and UCP-1 gene expression in brown adipocytes. Am. J. Physiol. Endocrinol. Metab. 2009; 296:E1430-9.
Sasaki T, Takemori H, Yagita Y, et al. SIK2 is a key regulator for neuronal survival after ischemia via TORC1-CREB. Neuron 2011; 69:106-19.
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