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CLEC2A Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q6UVW9 |
| Other Accession | NP_001124183, 195222725 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 19972 Da |
| Application Notes | CLEC2A antibody can be used for detection of CLEC2A by Western blot at 1 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 387836 |
|---|---|
| Target/Specificity | CLEC2A; CLEC2A antibody is human specific. Two alternatively spliced transcript variants have been observed. |
| Reconstitution & Storage | CLEC2A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | CLEC2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLEC2A |
|---|---|
| Synonyms | KACL |
| Function | Membrane-bound protein expressed mainly on keratinocytes which acts as a ligand to stimulate the activating receptor NKp65/KLRF2, expressed on the surface of natural killer (NK) cells (PubMed:25510854). Facilitates thereby dedicated immune recognition of keratinocytes leading to natural killer cell mediated cytotoxicity (PubMed:20194751). Also plays a role in modulating the extent of T-cell expansion (PubMed:18550855). |
| Cellular Location | Cell membrane; Single-pass type II membrane protein |
| Tissue Location | Mainly expressed in skin. Also expressed in keratinocytes, spleen, thymus, small intestine, peripheral blood monocytes, bone marrow, ovary, testis and skin. High expression in CD8(+), B-lymphocytes and naive CD4(+) T-cells. Restricted mostly to proliferating lymphocytes. Not detected in myeloid leukocytes or natural killer (NK) cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CLEC2A Antibody: CLEC2A (C-type lectin domain family 2 member A), also known as PILAR (proliferation-induced lymphocyte-associated receptor) is involved in modulating T-cell expansion. It is a 32 kDa single-pass type II membrane protein that contains one C-type lectin domain in its extracellular region and belongs to the CTL/CTLD superfamily. CLEC2A is mainly expressed in skin and highly expressed in CD8(+), B lymphocytes and naive CD4(+) T cells. Manipulation of CLEC2A signaling may be important for treatment of autoimmune diseases and cancer.
References
Huarte E, Cubillos-Ruiz JR, Nesbeth YC, et al. PILAR is a novel modulator of human T-cell expansion. Blood 2008; 112:1259-68.
Drickamer K. C-type lectin-like domains. Curr. Opin. Struct. Biol. 1999; 9:585-90
Spreu J, Kienle EC, Schrage B, et al. CLEC2A: a novel, alternatively spliced and skin-associated member of the NKC-encoded AICL-CD69-LLT1 family. Immunogenetics 2007; 59:903-12.
Spreu J, Kuttruff S, Stejfova V, et al. Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Proc. Natl. Acad. Sci. USA 2010; 107:5100-5.
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