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TCF7L2 Antibody

Rabbit Anti-Human TCF7L2 Polyclonal

     
  •  - TCF7L2 Antibody ASM10571
    Immunohistochemistry analysis using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.
    detail
  •  - TCF7L2 Antibody ASM10571
    Immunohistochemistry analysis using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession Q9NQB0
Other Accession NP_001139746.1
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format TCF7L2
Target/Specificity TCF7L2
Other Names Transcription factor 7-like 2 antibody, HMG box transcription factor 4 antibody, hTCF 4 antibody, hTCF-4 antibody, T cell factor 4 antibody, T cell specific HMG box antibody, T cell specific transcription factor 4 antibody, T-cell factor 4 antibody, T-cell-specific transcription factor 4 antibody, TCF 4 antibody, TCF-4 antibody, TCF4 antibody, TCF7L2 antibody, TCF7L2 protein antibody, TF7L2_HUMAN antibody, Transcription factor 7 like 2 antibody, Transcription factor 7 like 2 T cell specific HMG box antibody
Clone Names TCF7L2
Immunogen Synthetic peptide of Human TCF7L2 (400-500 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-722 was sufficient for detection of TCF7L2 in 15 µg of rat kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Pml Body
Research Areas
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$ 330.00
Cat# ASM10571
11 Formats Available
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