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GDNF Antibody

Rabbit Anti-Human GDNF Polyclonal

     
  • WB - GDNF Antibody ASM10706
    Western blot analysis with Stressmarq’s anti-GDNF polyclonal antibody (ASM10706) showing detection of human recombinant GDNF protein (SinoBiological 10561-HNAE). This recombinant protein has a predicted molecular weight of 15.2 kDa. Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (ASM10706) at 1:5000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 9.122 seconds.
    detail
  • WB - GDNF Antibody ASM10706
    Western blot analysis with Stressmarq’s anti-GDNF polyclonal antibody (ASM10706) showing detection of GDNF protein in rat brain lysate (prepared in-house). Rat GNDF protein with signal and pro-peptide segments has a predicted molecular weight of 23.6 kDa (UniProt Q07731). Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody (ASM10706) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 7.299 seconds. NOTE secondary antibody shows some non-specific binding in the control, but not to the GNDF protein.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P39905
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human GDNF Polyclonal
Target/Specificity GDNF
Other Names Glial cell line-derived neurotrophic factor Antibody, Astrocyte-derived trophic factor Antibody, Astrocyte derived trophic factor 1 Antibody, Glial cell derived neurotrophic factor Antibody, ATF 1 Antibody, ATF 2 Antibody, Glial Cell Line Derived Neurotrophic Factor Antibody, ATF Antibody, Atf Antibody, HSCR3 Antibody, Astrocyte derived trophic factor Antibody, ATF2 Antibody, GDNF_HUMAN Antibody, GDNF Antibody, Glial derived neurotrophic factor Antibody, ATF1 Antibody, gdnf Antibody, HFB1 GDNF Antibody, hGDNF Antibody
Immunogen Recombinant Human GDNF Protein, Full length (AA78-211)
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/mL of ASM10706 was sufficient for detection of GDNF in 15 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Cat# ASM10706
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