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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-CHOLINE OXIDASE (Alcaligenes species) (GOAT) Antibody   

Anti-CHOLINE OXIDASE (Alcaligenes species) (GOAT) Antibody

Choline Oxidase Antibody

     
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Product Information
Host Goat
Conjugate Unconjugated
Target Species Alcaligenes
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IP, I, LCI
Application Note Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:22,000 of the reconstitution concentration is suggested for this product.
Physical State Lyophilized
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Choline Oxidase [Alcaligenes species]
Reconstitution Volume 100 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Purity Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].  Cross reactivity against Choline Oxidase from other sources is unknown.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Name CHOX
Function Oxidizes choline to betaine, probably via two successive reactions with betaine aldehyde as an intermediate (PubMed:6997283). Accepts either choline or the reaction intermediate betaine-aldehyde as substrate (PubMed:6997283). Glycine-betaine accumulates to high levels in the cytoplasm of cells to prevent dehydration and plasmolysis in adverse hyperosmotic environments (By similarity).
Research Areas
Citations (0)
citation

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Background

Choline Oxidase catalytic activity is Choline + 2 O2 + H2O = betaine + 2 H2O2. This protein is involved in step 1 of the subpathway that synthesizes betaine from choline.

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$ 499.00
Cat# ASR3910
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