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> home > Products > Primary Antibodies > Signal Transduction > Anti-CHOLINE OXIDASE (Alcaligenes species) (GOAT) Antibody Biotin Conjugated
Anti-CHOLINE OXIDASE (Alcaligenes species) (GOAT) Antibody Biotin Conjugated
Choline Oxidase Antibody Biotin Conjugated
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Goat |
|---|---|
| Conjugate | Biotin |
| Target Species | Alcaligenes |
| Clonality | Polyclonal |
Application 
| WB, E, IP, I, LCI |
| Application Note | Anti-Choline Oxidase has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Choline Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Physical State | Lyophilized |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Choline Oxidase [Alcaligenes species] |
| Reconstitution Volume | 100 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Purity | Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown. |
|---|---|
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | CHOX |
|---|---|
| Function | Oxidizes choline to betaine, probably via two successive reactions with betaine aldehyde as an intermediate (PubMed:6997283). Accepts either choline or the reaction intermediate betaine-aldehyde as substrate (PubMed:6997283). Glycine-betaine accumulates to high levels in the cytoplasm of cells to prevent dehydration and plasmolysis in adverse hyperosmotic environments (By similarity). |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Choline Oxidase is a flavoprotein responsible for the catalyzing the conversion of choline to betaine aldehyde in animals and bacteria. Anti-Choline Oxidase antibody is ideal for researchers interested in Metabolism and Signal Transduction research.
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$ 480.00
Cat# ASR4020
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