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LCN1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant LCN1.

     
  • WB - LCN1 Antibody (monoclonal) (M02) AT2680a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.41 KDa) .
    detail
  • WB - LCN1 Antibody (monoclonal) (M02) AT2680a
    Western Blot analysis of LCN1 expression in transfected 293T cell line by LCN1 monoclonal antibody (M02), clone 1B11.

    Lane 1: LCN1 transfected lysate(19.25 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - LCN1 Antibody (monoclonal) (M02) AT2680a
    Detection limit for recombinant GST tagged LCN1 is approximately 3ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P31025
Other Accession NM_002297
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B11
Calculated MW 19250 Da
Additional Information
Gene ID 3933
Other Names Lipocalin-1, Tear lipocalin, Tlc, Tear prealbumin, TP, Von Ebner gland protein, VEG protein, LCN1, VEGP
Target/Specificity LCN1 (NP_002288, 24 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsLCN1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene belongs to the lipocalin family. Lipocalins are a group of extracellular proteins that are able to bind lipophiles by enclosure within their structures to minimize solvent contact. This protein may bind hydrophobic ligands and inhibit cysteine proteinases. It may also play a role in taste reception.

References

A new crystal form of human tear lipocalin reveals high flexibility in the loop region and induced fit in the ligand cavity. Breustedt DA, et al. Acta Crystallogr D Biol Crystallogr, 2009 Oct. PMID 19770509.Intracavitary ligand distribution in tear lipocalin by site-directed tryptophan fluorescence. Gasymov OK, et al. Biochemistry, 2009 Aug 4. PMID 19586017.Tear lipocalin is the major endonuclease in tears. Yusifov TN, et al. Mol Vis, 2008 Jan 29. PMID 18334931.Evidence for internal and external binding sites on human tear lipocalin. Gasymov OK, et al. Arch Biochem Biophys, 2007 Dec 1. PMID 17945179.Oligomeric state of lipocalin-1 (LCN1) by multiangle laser light scattering and fluorescence anisotropy decay. Gasymov OK, et al. Biochim Biophys Acta, 2007 Oct. PMID 17869594.

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$ 350.00
Cat# AT2680a
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Availability: 7-10 days
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