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RCV1 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant RCV1.

     
  • WB - RCV1 Antibody (monoclonal) (M05) AT3607a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - RCV1 Antibody (monoclonal) (M05) AT3607a
    Western Blot analysis of RCV1 expression in transfected 293T cell line by RCV1 monoclonal antibody (M05), clone 2H4.

    Lane 1: RCV1 transfected lysate(23.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - RCV1 Antibody (monoclonal) (M05) AT3607a
    Detection limit for recombinant GST tagged RCV1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P35243
Other Accession NM_002903.1
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2H4
Calculated MW 23130 Da
Additional Information
Gene ID 5957
Other Names Recoverin, Cancer-associated retinopathy protein, Protein CAR, RCVRN, RCV1
Target/Specificity RCV1 (NP_002894.1, 101 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRCV1 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.

References

Amino acid residues in GRK1/GRK7 responsible for interaction with S-modulin/recoverin. Torisawa A, et al. Photochem Photobiol, 2008 Jul-Aug. PMID 18266817.Establishment of a novel small cell lung carcinoma cell line with specific recoverin expression from a patient with cancer-associated retinopathy. Kobayashi M, et al. Lung Cancer, 2007 Jun. PMID 17374419.A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Functional analysis of the human recoverin gene promoter. Wiechmann A, et al. Curr Eye Res, 2003 Jan. PMID 12789533.

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$ 350.00
Cat# AT3607a
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Availability: 7-10 days
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