|Other Names||5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2014b was selected from the C-term region of human NT5E . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
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Provided below are standard protocols that you may find useful for product applications.
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003).Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002).Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990).Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988).Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
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