KD-Validated Anti-Helicase like transcription factor Rabbit Monoclonal Antibody

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18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

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Crown Products

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications.

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New Products

Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

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Current Promotion

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Research Focus

Greneral Collections

Peptides

SARS Peptides

Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.

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Synthetic Peptides

All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.

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Blocking Peptides

The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

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Cell/Tissues/Lysates

Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.

These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment.

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Citations

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

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Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q14527
Reactivity	Human
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone Names	23GB 2485
Calculated MW	Predicted, 114 kDa , observed, 120 kDa
Gene Name	HLTF
Aliases	HLTF; Helicase Like Transcription Factor; HIP116A; RNF80; SMARCA3; SNF2L3; HLTF1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 3; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 3; RING-Type E3 Ubiquitin Transferase HLTF; Sucrose Nonfermenting Protein 2-Like 3; Helicase-Like Transcription Factor; RING Finger Protein 80; HIP116; ZBU1; DNA-Binding Protein/Plasminogen Activator Inhibitor-1 Regulator; DNA-Binding Protein/Plasminogen Activator Inhibitor 1 Regulator; Sucrose Nonfermenting-Like 3; SNF2-Like 3; EC 2.3.2.27; EC 3.6.4.-; EC 3.6.1
Immunogen	A synthesized peptide derived from human HLTF

Gene ID	6596
Other Names	Helicase-like transcription factor, 2.3.2.27, 3.6.4.-, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1

Name	HLTF (HGNC:11099)
Function	Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT) (PubMed:18316726, PubMed:18719106, PubMed:26051180, PubMed:31960921, PubMed:39142279, PubMed:40680746). Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching (PubMed:18316726, PubMed:18719106). Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance: specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance (PubMed:40680746). Catalyzes formation of H3K23ub in two steps: first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2 (PubMed:40680746). Also acts as a chromatin redodeling factor, thereby regulating transcription (PubMed:10391891, PubMed:1994885, PubMed:9126292). Exhibits ATP-dependent double-stranded DNA (dsDNA) translocase activity but lacks classical helicase activity; mediates replication fork reversal by concertedly unwinding and annealing nascent and parental strands, thereby suppressing DNA synthesis and maintaining genomic stability (PubMed:1994885). Resolves G-quadruplex (G4) DNA structures in cooperation with MSH2, limiting replication stress and G4 accumulation across the cell cycle (PubMed:39142279). Contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides using its HIRAN and ATPase domains (PubMed:26051180). Can displace single-stranded DNA from triplex structures through ATP-dependent dsDNA translocation (PubMed:26051180, PubMed:31960921). Also has protein clearing activity at the stalled replication fork, facilitating restart of DNA replication (PubMed:21795603).
Cellular Location	Nucleus. Chromosome
Tissue Location	Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.

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