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KD-Validated Anti-Transcription Factor 7 Like 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Transcription Factor 7 Like 1 Rabbit Monoclonal Antibody AGI1706
    Western blotting analysis using anti-Transcription factor 7 like 1 antibody (Cat#AGI1706). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Transcription factor 7 like 1 antibody (Cat#AGI1706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Transcription Factor 7 Like 1 Rabbit Monoclonal Antibody AGI1706
    Western blotting analysis using anti-Transcription factor 7 like 1 antibody (Cat#AGI1706). Transcription factor 7 like 1 expression in wild type (WT) and Transcription factor 7 like 1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Transcription factor 7 like 1 antibody (Cat#AGI1706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Transcription Factor 7 Like 1 Rabbit Monoclonal Antibody AGI1706
    Flow cytometric analysis of Transcription factor 7 like 1 expression in HT-1080 cells using anti-Transcription factor 7 like 1 antibody (Cat#AGI1706, 1:2,000). Green, isotype control; red, Transcription factor 7 like 1.
    detail
  • ICC - KD-Validated Anti-Transcription Factor 7 Like 1 Rabbit Monoclonal Antibody AGI1706
    Immunocytochemical staining of HT-1080 cells with anti-transcription factor 7 like 1 antibody (Cat#AGI1706, 1:1,000). Nuclei were stained blue with DAPI; Transcription factor 7 like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9HCS4
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB1985
Calculated MW Predicted, 63 kDa ; Observed , 63 kDa
Gene Name TCF7L1
Aliases TCF7L1; Transcription Factor 7 Like 1; TCF3; Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box); HMG Box Transcription Factor 3; Transcription Factor 7-Like 1; TCF-3
Immunogen A synthesized peptide derived from human TCF3
Additional Information
Gene ID 83439
Other Names Transcription factor 7-like 1, HMG box transcription factor 3, TCF-3, TCF7L1, TCF3
Protein Information
Name TCF7L1
Synonyms TCF3
Function Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance.
Cellular Location Nucleus.
Tissue Location Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1706
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