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KD-Validated Anti-NEFM Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-NEFM Mouse Monoclonal Antibody AGI1923
    Western blotting analysis using anti-neurofilament medium chain antibody (Cat#AGI1923). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-neurofilament medium chain antibody (Cat#AGI1923, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-NEFM Mouse Monoclonal Antibody AGI1923
    Western blotting analysis using anti-neurofilament medium chain antibody (Cat#AGI1923). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-neurofilament medium chain antibody (Cat#AGI1923, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-NEFM Mouse Monoclonal Antibody AGI1923
    Flow cytometric analysis of Neurofilament medium chain expression in HeLa cells using anti-Neurofilament medium chain antibody (Cat#AGI1923, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P07197
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB5825
Calculated MW Predicted, 102 kDa, observed, 150 kDa
Gene Name NEFM
Aliases NEFM; Neurofilament Medium Chain; NF-M; NFM; NEF3; Neurofilament, Medium Polypeptide 150kDa; Neurofilament Medium Polypeptide; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; Neurofilament, Medium Polypeptide; Neurofilament-3 (150 KD Medium); Neurofilament Medium
Immunogen Recombinant protein of human NEFM
Additional Information
Gene ID 4741
Other Names Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM
Protein Information
Name NEFM
Synonyms NEF3, NFM
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Cellular Location Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P08553}. Cell projection, axon {ECO:0000250|UniProtKB:P08553}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1923
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