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SNAP29 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SNAP29.

     
  • IF - SNAP29 Antibody (monoclonal) (M01) AT3973a
    Immunofluorescence of monoclonal antibody to SNAP29 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - SNAP29 Antibody (monoclonal) (M01) AT3973a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.12 KDa) .
    detail
  • WB - SNAP29 Antibody (monoclonal) (M01) AT3973a
    Western Blot analysis of SNAP29 expression in transfected 293T cell line by SNAP29 monoclonal antibody (M01), clone 3E4-E6.

    Lane 1: SNAP29 transfected lysate(28.38 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - SNAP29 Antibody (monoclonal) (M01) AT3973a
    Detection limit for recombinant GST tagged SNAP29 is 0.1 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O95721
Other Accession BC009715
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3E4-E6
Calculated MW 28970 Da
Additional Information
Gene ID 9342
Other Names Synaptosomal-associated protein 29, SNAP-29, Soluble 29 kDa NSF attachment protein, Vesicle-membrane fusion protein SNAP-29, SNAP29
Target/Specificity SNAP29 (AAH09715, 1 a.a. ~ 258 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSNAP29 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.

References

1.SNARE protein expression and localization in human cytotoxic T lymphocytes.Pattu V, Qu B, Schwarz EC, Straubeta B, Weins L, Bhat SS, Halimani M, Marshall M, Rettig J, Hoth M.Eur J Immunol. 2011 Nov 25. doi: 10.1002/eji. 201141915.

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$ 350.00
Cat# AT3973a
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